In vitro Antioxidant Activity of Extracts of Psidium guajava Leaves

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Pharmacology, Toxicology and Biomedical Reports,2017,3,3,50-54.
Published:January 2018
Type:Original Article

In vitro Antioxidant Activity of Extracts of Psidium guajava Leaves

Ramasamy Manikandan1*, Arumugam Vijaya Anand2, Rengasamy Lakshminarayanan Rengaraj3, Puthamohan Vinayakamoorthi2, Radhakrishnan Ramalingam4

1Department of BioChemistry, M.I.E.T Arts and Science College, Trichirappalli-620 007, Tamil Nadu, INDIA.

2Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore-641 046, Tamil Nadu, INDIA.

3Centre for Pheromone technology, Department of Animal sciences, Bharathidasan University, Trichy, Tamil Nadu, INDIA.

4School of Biotechnology, Yeungnam University, SOUTH KOREA.


Background: The incidence of diabetes is increasing rapidly in world wide. It produces disturbances in the metabolism of carbohydrate, protein, and lipid. The reports prove that the various oxidative reactions are responsible for the various disorders like diabetes, cancer, aging etc. Evidences recommended that the natural medicines originating from plant source may represent a culturally relevant complementary treatment for diabetes. The present study aims to investigate the antioxidant activity of P. guajava leaves in an in vitro model of various extracts such as aqueous, ethanol and methanol. Method: The scavenging activity of DPPH, reducing power assay, NO, H2O2 and SOD is determined. Results: The results of the present study, proves all the three extracts which have an antioxidant activity in a dose dependent manner (250, 500, 750, 1000, 1500 μg/ml). The ethanolic extract of P. guajava leaves which have high activity when compared to the other two extracts. Conclusion: All the three extracts of P. guajava leaves have an antioxidant activity in an in vitro model. Among the various extracts, the ethanolic extract has a high activity. Further studies are needed for compound identification and its action in an in vivo model