Isolation of Lactobacillus sp. from Intestinal Tract of Red Jungle Fowl as an Effective Probiotic for Poultry

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Abstract
Pharmacology, Toxicology and Biomedical Reports,2018,4,2,15-18.
Published:September 2018
Type:Case Report

Isolation of Lactobacillus sp. from Intestinal Tract of Red Jungle Fowl as an Effective Probiotic for Poultry

S Santhosh, R Ranjith Kumar, R Dhandapani*

Department of Microbiology, School of biosciences, Periyar University, Salem, Tamil Nadu, INDIA.

Abstract:

Objective: The present study focuses on the use of Lactobacillus sp. as probiotics to the Red jungle fowl and to test its growth efficacy. Materials and Methods: The isolated colonies were optimized at different pH range and it was found that at pH 7 maximum O.D value of 0.725 was attained and that of Temperature optimization at 30°C showed a maximum O.D value at 0.624 when read at 600 nm. The optimized pH and temperature were used for the mass production of lactobacillus sp. for 3 days in orbital shaker and the biomass. The Biomass was fed to one month grown red jungle fowls , the biomass was mixed with commercial feed and the weight of the fowls were checked every 4 days interval to a total of 28 days. Results: A total of five colonies were isolated from the serial dilution performed on the nutrient agar medium and then it was streaked on MRS a specific medium for Lactobacillus sp. The isolated Lactobacillus sp. was given as a probiotic to the two test fowls and the weight of the fowls was 295 g on the 4th day and which increased gradually to 595 g on the 28th day. This shows that bacteria could be