Purpose: To explore if the factors (gender, age, social status and income) affect the job satisfaction of pharmacy career in Saudi Arabia. Methods: This is a 4-month cross sectional survey regarding the impact of stress factors on job satisfaction of a pharmacist in Saudi Arabia. The survey questionnaire consisted of 35 questions divided into two parts: first part collected demographic information and the second part consisted on various sections. Section one collected information about stress factors in pharmacy job. Section two collected information about service units of the pharmacy job. Section three collected information about communication and relationship factors of a pharmacy job. Factors affected the Pharmacy practice, stress-related job and communication and relationships sections of pharmacist job satisfactions including gender, age, social status, academic qualifications and income. The questionnaire was prepared in an electronic format and the data were analyzed through the Survey Monkey system. Results: A total of 242 participants responded to the questionnaire. Of them, 214 (91.8%) were Saudi and 33 (8.2%) were non-Saudi professionals. Male responders were more than female responders (169 (70.7%) and 70 (29.3%), respectively). The majority of the responders (202 (84.2%)) were in the age group of 20–39 years. Females disagreed management policies more than that of males; males worked on many weekends and on holidays with significant differences when compared with females (p<0.05). Responders in the age group of 30–39 years strongly agreed to perform excessive work than those in the age group of 20–29 years, which was found to be significant (p<0.05). Both males and females wished to continue to work in the pharmacy field, which was not significantly different (p>0.05). There is no significant difference between male and female with all level of salary and type work with payment or the salary resemble of other organizations (p>0.05). Conclusion: Different factors were found to negatively and positively affect the job satisfaction of a pharmacist. Gender and age were found to affect job satisfaction significantly. These differences should be considered before interpreting the results of pharmacist job satisfaction. We recommend to improve the job satisfaction of pharmacists in the Kingdom of Saudi Arabia.