Written by Pharmacology, Toxicology and Biomedical Reports,2015,1,2,37-40.
DOI: 10.5530/PTB.1.2.1
Published: 15th May 2015
Type: Methods
Authors: Subramani Parasuraman, Khor Ming Zhen, and Ramasamy
Author(s) affiliations:
Subramani Parasuraman*1, Khor Ming Zhen1, Ramasamy Raveendran2
1Unit of Pharmacology, Faculty of Pharmacy, AIMST University, 08100 Bedong, Kedah, Malaysia
2Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India
Abstract
Rats are the most commonly used laboratory animals for wide range of scientific research. Collection of biological fluids (such as blood, gastric fluid, breast milk and urine) is one of the important step in experiments. In many experiments, blood samples are collected for analysis of biochemical parameters and genetic profile. Blood samples are commonly collected through the tail vein, retro-orbital sinus and cardiac puncture. Retro-orbital sinus is commonly employed for collecting large volume of blood (200 μL to 1 mL) and it is employed in terminal procedures too. The main objective of this paper is to discuss the general principles of blood sample collection and the method used for orbital sinus blood sample collection.