@article {27, title = {Dexamethasone-Loaded PLGA Microspheres: Effective and Sustained Anticancer Drug Delivery System}, journal = {Pharmacology, Toxicology and Biomedical Reports}, volume = {3}, year = {2017}, month = {September 2017}, pages = {55-61}, type = {Original Article}, chapter = {55}, abstract = {

Objective: Encapsulation of dexamethasone (DEX) anticancer drug with Poly (d, l-lactide-co-glycolide) (PLGA) microsphere (MS) was carried out for sustained release system. Methods: The characterizations of MS were analyzed using XRD, FTIR, RP-HPLC, TEM, CLSM, and XRD patterns and reported the physical state of the encapsulated PLGA microsphere (DEXPLGA MS). Results: The presence of 2 θ peaks values at 33.17{\textdegree}, 35.63{\textdegree}, 49.46{\textdegree} and 54.09{\textdegree} confirmed the semi-crystalline phase for the DEX drug. FTIR showed peaks at 1695 cm-1, 1644 cm-1 and 1602 cm-1 were shown to be confirming DEX encapsulation. The MTT assay showed a greater cellular uptake of DEX-PLGA MS and the new formulation showed steady and sustained release of DEX resulting in a lower short term cytotoxic effect against HEP-G2 cells. Conclusion: In the present study, the drug encapsulation microsphere is non-toxic, simple, and an ideal method for anticancer drug release. The present results revealed that the use of DEX-PLGA MS offers large-scale production of biocompatible microspheres that can be used against HEP-G2 cells.

}, keywords = {Confocal laser scanning microscopy, Dexamethasone, MTT assay, PLGA, Sustained release, TEM}, doi = {10.5530/PTB.2017.3.11}, author = {Elangovan Vimalkumar and Chinnaperumal Kamaraj and Pachiyappan Rajiv Gandhi} }