@article {29, title = {GC-MS Metabolite Profiling, Antibacterial, Antidiabetic and Antioxidant Activities of Brown Seaweeds, Sargassum wightii Greville Ex J. Agardh, 1848 and Stoechospermum marginatum (C. Agardh) K{\"u}tzing 1843}, journal = {Pharmacology, Toxicology and Biomedical Reports}, volume = {3}, year = {2017}, month = {October 2017}, pages = {27-34}, type = {Original Article}, chapter = {27}, abstract = {

Objectives: The present study focuses on antibacterial, antidiabetic and antioxidant activities of methanolic extracts of brown seaweed Stoecho- spermum marginatum (SMME) and Sargassum wightii (SWME). Methods: The antibacterial activities of the seaweed extracts were determined by agar well diffusion method. Phytochemical, antioxidant and antidiabetic activity of the selected seaweed extracts were performed. Results: The phytochemical analysis of SMME and SWME has confirmed the presence of fixed oil, fat, tannin, flavonoids, alkaloids, steroids, phenol compounds, saponin etc. The SWME showed maximum activity against Staphylococ- cus aureus and minimum activity against Escherichia coli. And the SMME has shown moderate activity against S. aureus. The antidiabetic efficacy of SWME revealed the maximum effect with the inhibitory concentration value (IC50: 58.36 μg/mL) followed by SMME. The SWME has showed the highest scavenging property in all the tested assays (DPPH, FRAP and H2O2) in relation to the control, ascorbic acid. The bioactive metabolites of the extracts were chemically characterized by FTIR and GCMS analyses. GC-MS analysis of SWME revealed the presence of a major chemical compound, hexadecenoic acid, methyl ester (13.35\%) which might be responsible for the recorded activity. The FTIR spectrum analyses of crude extracts revealed the presence of alkyl halides, alkanes, amides, aromatics and carboxylic acids. Hence, the present study could form a base-line for the effective biomedical utilization of the seaweed, S. wightii.

}, keywords = {Antidiabetic, Antioxidant, Brown seaweeds, Sargassum wightii, Stoechospermum mariginatum.}, doi = {10.5530/PTB.2017.3.5}, url = {http://fulltxt.org/article/349}, author = {Paramasivam Deepak and Muthu Paulraj Diviya Josebin and Rajasekaran Kasthuridevi and Rajamani Sowmiya and Govindasamy Balasubramani and Dilipkumar Aiswarya and Pachiappan Perumal} } @article {40, title = {Antihypergluco-lipidemic and Antioxidant activities in Aqueous methanol Extract of Some Vegetables Peel: An in vitro Analysis}, journal = {PTB Reports}, volume = {2}, year = {2016}, month = {Dec 2015}, pages = {15-20}, type = {Research Aricle}, chapter = {15}, abstract = {

Objective: To examine phytochemical constituents, antihyperglycemic, antihyperlipidemic and free radical scavenging activities in 50\% methanol extract of peels of Cucumis melo var. chito (CM), Cucumis pubescens (CP), Lagenaria siceraria (LS), Luffa acutangula (LA), and Momordica charantia (MC) applying in vitro methods. Material and Methods: Total polyphenol (gallic acid equivalent; GAE), flavonoid (rutin equivalent; RE) and anthocyanin (cyanidine-3-glucoside equivalent; CGE) content were estimated. The 2,2-diphenyl-1-picrylhydrazyl (DPPH); 2,2-azinobis(3-ethylbenzthiazoline- 6-sulphonic acid) (ABTS.+); nitric oxide (NO) scavenging; nitro blue tetrazolium (NBT) reduction; advanced glycation end-products (AGEs), intestinal α-glucosidase, pancreatic lipase and liver Protein-tyrosine phosphatase 1β (PTP 1β) inhibitory activities were examined in vitro. Results: Peel of CM (1190 μgGAE/g) and MC (923 μgGAE/g) was rich in polyphenol and flavonoids (68.8, 145.1 μgRE/g respectively). Anthocyanin content was more in MC (167 mg CGE/100 g) than others. LS potently scavenged ABTS.+ (SC50, 99 μg) and DPPH (SC50 , 0.6 mg) radicals. Inhibition of vesperlysinelike AGEs (71.5\%) was significant (p\<0.006) than pentosidine-like AGEs (14.8\%) by LA peel. Inhibition of pentosidine-like AGEs was significant (p\<0.009) by CM than CP peel. MC reduced (84.5 \%) only vesperlysine-like AGEs. Intestinal α-glucosidase was inhibited (23.02 \%) by MC peel only. Pancreatic lipase was inhibited by CM (74.5\%) and CP (58.6 \%) peels. The LS reduced PTP 1β (32.1 \%) more potently than others. Conclusion: Vegetable peels present variable degree of antioxidant, antihyperglycemic and antihyperlipidemic activities.

}, keywords = {Advanced glycation end products, Antioxidant, Intestinal α-glucosidase, Porcine pancreatic lipase, Protein-tyrosine phosphatase 1β.}, doi = {10.5530/PTB.2016.1.4}, author = {Srishti Shukla and Ambati Anusha and Katta Archana and Dommati Anand Kumar and Amtul Zehra and Yellu Narsimha Reddy and Ashok Kumar Tiwari} }