02642nas a2200277 4500008004100000245014100041210006900182260001500251300000900266490000600275520174200281653001802023653001402041653002502055653002802080653001702108100001902125700002802144700002502172700002302197700002802220700002202248700001602270700001802286856006002304 2017 eng d00aElucidation of In-vitro thrombolytic, membrane stabilizing, cytotoxic activities and phytochemical nature of Bruguiera cylindrica leaves0 aElucidation of Invitro thrombolytic membrane stabilizing cytotox cApril 2017 a7-130 v33 a

Objectives: The present study was intended to investigate possible phytochemical nature (group determinant of plant extract) and to reveal in-vitro thrombolytic, membrane stabilizing and cytotoxic activities of crude methanolic extracts of Bruguiera cylindrica leaves. Methods: Collected plant parts were cleaned, dried, and ground to obtain powdery mass. Various methanolic extracts of B. cylindrica was subjected for evaluating membrane stabilizing potentials at a hypotonic solution and heat induce condition, where thrombolytic activity assessment was done by employing Streptokinase as standard. Finally, Cytotoxicity activity was determined by Brine shrimp Lethality bioassay. Results: Phytochemical screening of methanolic extracts revealed the presence of carbohydrate(s), glycoside(s), phenol, tannin, protein(s), gum and mucilages. Crude methanolic extracts with different doses were used for in-vitro thrombolytic study and 10 mg/ml concentration of plant extracts showed moderate clot lysis (14.51±1.87%) while the standard (streptokinase) showed 59.73 ±0.97% clot lysis. The methanolic extracts exhibited significant anti-inflammatory properties at 10mg/ml concentration by both hypotonic solution and heat induced haemolysis of erythrocyte membrane i.e. 18.53±0.91% and 23.60±0.89% respectively. In contrast to vincristine sulphate the crude methanolic extracts showed moderate cytotoxic properties with LC50 value of 16.628. Conclusions: Our present study suggest that, further studies may carry on this plant extracts to build up an acceptable report in the medical science.

10aB. cylindrica10aCytotoxic10aMembrane stabilizing10aphytochemical screening10aThrombolytic1 aIslam, Safiqul1 aMillat, Md., Shalahuddi1 aHussain, Md., Saddam1 aRahman, Md., Abdur1 aMoghal, Md., Mizanur Ra1 aNipa, Jasmin, Ara1 aHasan, Imam1 aHossain, Meer uhttps://ptbreports.org/article/2017/3/1/105530ptb201732